By Alton Meister
Advances in Enzymology and comparable parts of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative experiences of the newest discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, delivering an unequalled view of the old improvement of enzymology. The sequence deals researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic approach, and their program in either the laboratory and undefined. every one quantity within the sequence good points contributions via major pioneers and investigators within the box from worldwide. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.
With its wide selection of issues and lengthy old pedigree, Advances in Enzymology and similar components of Molecular Biology can be utilized not just by means of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally via any scientist drawn to the invention of an enzyme, its houses, and its purposes.
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Additional info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 21
The former is a very important property of mitochondria, because these granules do possess a powerful ATPase (26,179) which can be activated by a variety of treatments, and because development of this ATPase activity can lead to the induction of oxidation by mitochondria. This is believed to be due to the fact that ADP is the primary phosphate acceptor and that the concentration of this compound controls the level of mitochondrial oxidations (115). Since the development of the latent mitochondrial ATPase MITOCHONDHIAL METABOLISM 31 would result in an increased level of ADP, oxidation of substrate would ensue.
More recent experiments by these workers with a similar system showed that cholesterol was degraded to a complex mixture of products which included at least four acidic derivatives and a neutral derivative believed to be either 25- or 26-hydroxycholesterol (147-149). The hydroxylation of other MITOCHONDRIAL METABOLISM 27 steroids may also be catalyzed by mitochondria. Hayano and Dorfman (150) reported that an insoluble fraction of beef adrenal, sedimentable a t low speed, was capable of hydroxylating deoxycorticosterone a t carbon 11 to yield corticosterone, and Sweat (151) independently demonstrated that the reaction was catalyzed only by the mitochondrial fraction of this tissue.
Evidence was presented to show that oxidation of the saturated hydrocarbon ring followed formation of the CoA derivative and preceded conjugation with glycine. I n view of the fact that the mechanisms of the formation of these compounds has now been established, the localization of the enzymes involved requires reinvestigation to determine whether all of the component enzymes of the system are actually restricted to the mitochondria. MTTOCHONDRIAL METABOLISM 43 C. SYNTHESIS OF CITRULLINE AN11 RE,T,ATKI) COMPOUN1)S According to the classical work of Krebs and Henseleit (274), tho formation of urea in liver slices involves three steps: ( 1 ) the formation of citrulline from ornithine, (2) the conversion of citrulline to arginine, and (3) the hydrolysis of arginine to urea and ornithine.